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Mahanta, R.
- Cellulase Activity and Kinetics in Rice Grasshopper Hieroglyphus banian (Orthoptera: acrididae)
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Authors
Affiliations
1 Department of Zoology, Handique Girls’ College, Assam, Pin code-781001, IN
2 Department of Zoology ,Gauhati University, Assam, Pin code-781014, IN
3 Department of Zoology, Cotton College, Assam, Pin code-781001, IN
1 Department of Zoology, Handique Girls’ College, Assam, Pin code-781001, IN
2 Department of Zoology ,Gauhati University, Assam, Pin code-781014, IN
3 Department of Zoology, Cotton College, Assam, Pin code-781001, IN
Source
Indian Journal of Science and Technology, Vol 5, No 12 (2012), Pagination: 3753-3757Abstract
Grasshopper (Hieroglyphus banian), an orthopteran species is considered to be a serious pest of rice plant in India. To study the activity and kinetics of cellulase in grasshopper this experiment is undertaken and accordingly foregut and midgut homogenates were prepared. The cellulase enzyme activity and kinetics was measured by DNSA method of Miller,(1959) by taking different concentrations of crystalline cellulose substrate in spectrophotometer at 540nm. Cellulase activity in foregut and midgut were found to be 0.482 u/mg of tissue and 0.687u/mg of tissue respectively. Km values were determined separately both in foregut and midgut and were found to be 3 mg/dl and 2 mg/dl respectively. Experimental data indicates the presence of high cellulolytic activity in the midgut which may suggest that cellulases of endogenous origin are present in this organism. It can be expected that in future, detailed study of these efficient lignocellulolytic systems will help in identification of novel enzymes possessing features that optimize biotechnological applications for the biofuel industry. Moreover, identification of crucial insect cellulases may help in the development of insecticidal technologies aimed at inhibiting their vital digestive role. Considering that this grasshopper species is a pest of paddy and grass, characterization of insect cellulolytic systems may aid in reducing the grasshopper pest attack in Indian subcontinent.Keywords
Cellulase, Kinetics, Hieroglyphus banian, Foregut, MidgutFull Text
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- On the Nature of Laser Induced Fluorescence Spectrum of a Bore-Hole Sample of Coal
Abstract Views :490 |
PDF Views:294
Authors
Affiliations
1 Department of Chemistry, Dibru College, Dibrugarh-786003, Assam, IN
2 Department of Physics, Dibru College, Dibrugarh-786003, Assam, IN
3 Centre for Laser and Optical Science, New Uchamati, Doom Dooma-786151, Assam, IN
1 Department of Chemistry, Dibru College, Dibrugarh-786003, Assam, IN
2 Department of Physics, Dibru College, Dibrugarh-786003, Assam, IN
3 Centre for Laser and Optical Science, New Uchamati, Doom Dooma-786151, Assam, IN
Source
Indian Journal of Energy, Vol 1, No 3 (2012), Pagination: 1-2Abstract
The present work describes the fluorescence spectrum of a sample of coal recovered from a depth of about 2km from the surface as a bore hole sample by Oil India Limited at Duliajan .The fluorescence spectrum was excited with the help of a 25mW green diode pumped solid state laser (532nm).The characteristic feature of the spectrum is a diffuse band in the region 6500-5900AKeywords
Laser Induced Fluorescence, 25mW Green Diode, Bore-hole Sample of CoalFull Text
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